A University of Michigan spin-off company has developed a promising Covid-19 antibody test. The press release states:
ANN ARBOR—COVID-19 antibody testing that’s portable, fast,
cheap and highly precise—four attributes that are usually mutually
exclusive—could be possible with a microfluidic device invented at the
University of Michigan and developed by U-M startup Optofluidic Bioassay.
A microfluidic device, or “lab on a chip,” shrinks multiple
lab functions onto a single chip just millimeters or centimeters in size. The
technology enables faster results with smaller sample sizes.
The new system is believed to be the first microfluidic
approach to a gold standard testing protocol known as “enzyme-linked
immunosorbent assay,” or ELISA. The U-M researchers have previously published
results showing that their device can work as well as the slower, larger,
standard ELISA setup. They are currently validating it for use on COVID-19
antibodies.
They have demonstrated that they can detect synthetic
COVID-19 antibodies and they’re working with researchers at a hospital in New
Jersey on experiments with human blood from COVID-19 patients.
University and industry labs around the U.S. are clambering
to develop better antibody tests. The U-M approach is different in that it
centers on a device, rather than the chemical mixtures, or reagents, to detect
COVID-19 markers.
“We are unique because we are a hardware company,” said
Xudong (Sherman) Fan, U-M biomedical engineering professor and co-founder of
Optofluidic Bioassay. “Anyone working on COVID-19 antibody tests can use their
reagents in our device.”
Why we need antibody tests
Rapid and accurate antibody tests could play an important
role as governments, medical workers, scientists and private citizens alike
continue to navigate the pandemic, the researchers say. Antibody tests can
reveal who has already been exposed to the virus and developed immunity, at
least temporarily, and can safely go back to work. If done in a widespread way,
they could show the true scale of the pandemic and its death rate.
And the U-M researchers say their particular approach could
give doctors critical, near-real-time insights into how a patient is responding
to treatment, or a vaccine once one is developed.
Small-scale antibody testing has been done in some countries.
Research projects are underway in the U.S. and while kits are beginning to
materialize on the market here, they’re not yet widely available.
Antibody, or “serology” tests are different from the “PCR”
tests being used to diagnose COVID-19. Rather than screen for the virus itself,
serology tests detect antibodies—proteins the immune system manufactures to
fight it.
Microfluidic ELISA in the landscape of COVID-19 antibody
tests
The majority of labs working on serology kits are making a
particular type called “rapid diagnostic tests” that give a yes or no reading.
These are quick, but they have drawbacks. False positives can be a problem. And
because they don’t give a lot of information, they aren’t useful in monitoring
the immune system’s response during treatment.
A handful of labs are making ELISA tests. These are typically
quantitative and accurate, showing the concentration of antibodies. That makes
them more reliable and less prone to false positives than the rapid diagnostic
tests. But standard ELISA results take several hours, and the machines that
provide them are the size of refrigerators. In addition, the sample needs to be
sent to the test lab for analysis.
But microfluidic ELISA can give a quantitative and accurate
result in just 15 minutes, with a finger-prick’s worth of blood. This
combination of attributes, plus the fact that it’s portable, could make it a
powerful tool.
“Our approach offers the best of both worlds. We can achieve
the quickness and simplicity of the rapid diagnostic test with the accuracy of
the standard ELISA quantitative measure,” Fan said.
“Because our device generates such sensitive and quantitative
measurements, we believe its use goes beyond identifying recovered patients.
Antibodies begin to show up a few days after infection, so we could use this
approach to monitor patients’ immune response to infection, treatment and
vaccination.”
The microfluidic ELISA is rapid, portable and low cost.
“The estimated cost of testing is a few dollars per test of
two to three different antibodies, making this a very viable option for use in
hospitals, doctors’ offices, field clinics and potentially even pharmacies,”
said Xiaotian Tan, a doctoral student in biomedical engineering who is working
on COVID-19 antibody testing with Fan.
The machine can be the size of a microwave, and can test
multiple simultaneous samples of little more than a drop of blood from a
fingertip in less than 20 minutes.
It was invented at U-M several years ago and developed by
Optofluidic Bioassay, which was founded by Fan and former research investigator
of biomedical engineering, Maung (Malcolm) Khaing Oo, who now serves as the
company’’ chief technology officer. Fan and Maung Khaing Oo are co-founders of
and have an equity interest in Optofluidic Bioassay. The researchers plan to
eventually apply for FDA Emergency Use Authorization.
